Architectural Analysis of Picrosirius Red Stained Collagen in Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma using Polarization Microscopy
Published: December 1, 2015 | DOI: https://doi.org/10.7860/JCDR/2015/.6872
Rashi Sharma, Shweta Rehani, Monica Mehendiratta, Priyanka Kardam, Madhumani Kumra, Yulia Mathias, Jyoti Yadav, Khushboo Sahay
1. Senior Lecturer, Department Oral Pathology, Sudha Rustagi College of Dental Sciences and Research, Faridabad, Haryana, India.
2. Reader, Department of Oral Pathology, Sudha Rustagi College of Dental Sciences & Research, Faridabad, Haryana, India.
3. Reader, Department of Oral Pathology, ITS Dental College, Greater Noida, Uttar Pradesh, India.
4. Senior Lecturer, Department of Oral Pathology, Sudha Rustagi College of Dental Sciences & Research, Faridabad, Haryana, India.
5. Professor, Department of Oral Pathology, Sudha Rustagi College of Dental Sciences and Research, Faridabad, Haryana, India.
6. Professor and Head, Department of Oral Pathology, Sudha Rustagi College of Dental Sciences & Research, Faridabad, Haryana, India.
7. Senior Lecturer, Department of Oral Pathology and Mirobiology, Sudha Rustagi College of Dental Sciences and Research, Faridabad, India.
8. Senior Lecturer, Department of Oral Pathology, Sudha Rustagi College of Dental Sciences and Research, Faridabad, H
Correspondence
Dr. Priyanka Kardam,
D – 81, Ground Floor, Saket, New Delhi - 110017, India.
E-mail: priyankakardam@gmail.com
Introduction: Collagen degradation is important both for carcinogenesis and in its progression. Research regarding the co-relation of collagen with Oral Epithelial Dysplasia (OED) and Oral Squamous Cell Carcinoma (OSCC) is less explored.
Aim: To elucidate the nature of collagen in Oral Epithelial Dysplasia (OED) and Oral Squamous Cell Carcinoma (OSCC) using Picrosirius Red Stain (PSR) under polarizing microscopy. Materials and Methods: The study consisted of a total 40 samples which were divided into three groups. Group I included buccal mucosa as negative and irritation fibroma as positive control, group II consisted of OED and group III consisted of Oral Squamous Cell Carcinoma (OSCC). A histochemical analysis was conducted using PSR-polarization method by two independent observers.
Results: The control group shows predominantly reddish–orange birefringence. In OED with the advancement of grades, the colour changed from yellowish-orange colour to yellow-greenish with progressive increase in greenish hue. As OSCC regresses from well to poorly differentiated, the colour changed from reddish-orange to yellowish orange to greenish-yellow suggesting a transition from mature to immature collagen.
Conclusion: An observable gradual change in collagen of both OED and OSCC was noted as they were proceeding from benign to critical step. Thus, PSR is a useful tool for studying stromal changes as supporting collagen shows the transition in the form besides the alterations in epithelial cells.
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